tagH [2019-08-12 08:49:35]

[SW|ABC transporter] for teichoic acid translocation (ATP-binding protein)

locus: BSU_35700

pI: 9.45

mw: 59.07 kDa

protein length: 527 aa

gene length: 1584 bp

function: [category|SW 2.6.1.3|Biosynthesis of teichoic acid]

product: [SW|ABC transporter] for teichoic acid translocation (ATP-binding protein)

essential: yes

ec: 3.6.3.40

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.6|Export of anionic polymers and attachment to peptidoglycan]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.4|Export of cell wall components]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,673,564 3,675,147

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + teichoic acid(In) = ADP + phosphate + teichoic acid(Out) (according to Swiss-Prot)

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Structure

• [PDB|5WS4] (from Acinetobacter baumannii, corresponds to aa 29 ... 353, 23% identity)

[SW|Localization]

• membrane associated (via [protein|6795F1703416DCD04E51C177F1C9FE45E73ABAEF|TagG]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|8620DA76A1953D7ACDFA7E46ED81EF1A0D97999C|tagD]-[gene|88028A5F3520CB6DAB014412974EB59CC5D226DD|tagE]-[gene|E730E41546298960A4B907FCB7F36C495AA9602B|tagF]-[gene|6795F1703416DCD04E51C177F1C9FE45E73ABAEF|tagG]-[gene|4D3C316965834130BB378CBD1265CFA13F086C7A|tagH]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7581998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: repression, [Pubmed|9457886], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|12950927], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • repressed by phosphate ([protein|search|PhoP]) [Pubmed|9457886]

Biological materials

GFP fusion

• 3110 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|4D3C316965834130BB378CBD1265CFA13F086C7A|tagH]::pSG5050 (cat P_xyl-gfp-[gene|4D3C316965834130BB378CBD1265CFA13F086C7A|tagH]1-648) [pubmed|18156271], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

Reviews

• 24024634

Original publications

• 18156271,10092453,7565096,21280653,22383849,29877791